DNA
MHETrify-2

Part:BBa_K4625069:Design

Designed by: Tanishque Verma   Group: iGEM23_VITVellore   (2023-10-11)


MHETaseS196A,T434S


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 559
    Illegal XhoI site found at 1817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 814
    Illegal AgeI site found at 311
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pelB signal sequence has been incorporated for extracellular production of the MHETase gene


Source

Ideonella sakaiensis, synthetic

References